Fungal resistance mediated by maize wall-associated kinase ZmWAK-RLK1 correlates with reduced benzoxazinoid content.
- Yang P Department of Plant and Microbial Biology, University of Zurich, Zollikerstrasse 107, CH-8008, Zurich, Switzerland.
- Praz C Department of Plant and Microbial Biology, University of Zurich, Zollikerstrasse 107, CH-8008, Zurich, Switzerland.
- Li B Institute of Plant Sciences, University of Bern, CH-3013, Bern, Switzerland.
- Singla J Department of Plant and Microbial Biology, University of Zurich, Zollikerstrasse 107, CH-8008, Zurich, Switzerland.
- Robert CAM Institute of Plant Sciences, University of Bern, CH-3013, Bern, Switzerland.
- Kessel B KWS SAAT SE, DE-37574, Einbeck, Germany.
- Scheuermann D KWS SAAT SE, DE-37574, Einbeck, Germany.
- Lüthi L Department of Plant and Microbial Biology, University of Zurich, Zollikerstrasse 107, CH-8008, Zurich, Switzerland.
- Ouzunova M KWS SAAT SE, DE-37574, Einbeck, Germany.
- Erb M Institute of Plant Sciences, University of Bern, CH-3013, Bern, Switzerland.
- Krattinger SG Department of Plant and Microbial Biology, University of Zurich, Zollikerstrasse 107, CH-8008, Zurich, Switzerland.
- Keller B Department of Plant and Microbial Biology, University of Zurich, Zollikerstrasse 107, CH-8008, Zurich, Switzerland.
- 2018-09-05
Published in:
- The New phytologist. - 2019
Htn1
benzoxazinoids (BXs)
maize disease resistance
receptor-like kinase
wall-associated kinase (WAK)
Ascomycota
Benzoxazines
Disease Resistance
Plant Diseases
Zea mays
English
Wall-associated kinases (WAKs) have recently been identified as major components of fungal and bacterial disease resistance in several cereal crop species. However, the molecular mechanisms of WAK-mediated resistance remain largely unknown. Here, we investigated the function of the maize gene ZmWAK-RLK1 (Htn1) that confers quantitative resistance to northern corn leaf blight (NCLB) caused by the hemibiotrophic fungal pathogen Exserohilum turcicum. ZmWAK-RLK1 was found to localize to the plasma membrane and its presence resulted in a modification of the infection process by reducing pathogen penetration into host tissues. A large-scale transcriptome analysis of near-isogenic lines (NILs) differing for ZmWAK-RLK1 revealed that several differentially expressed genes are involved in the biosynthesis of the secondary metabolites benzoxazinoids (BXs). The contents of several BXs including DIM2 BOA-Glc were significantly lower when ZmWAK-RLK1 is present. DIM2 BOA-Glc concentration was significantly elevated in ZmWAK-RLK1 mutants with compromised NCLB resistance. Maize mutants that were affected in overall BXs biosynthesis or content of DIM2 BOA-Glc showed increased NCLB resistance. We conclude that Htn1-mediated NCLB resistance is associated with a reduction of BX secondary metabolites. These findings suggest a link between WAK-mediated quantitative disease resistance and changes in biochemical fluxes starting with indole-3-glycerol phosphate.
- Language
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- English
- Open access status
- bronze
- Identifiers
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- DOI 10.1111/nph.15419
- PMID 30178602
- Persistent URL
- https://sonar.rero.ch/global/documents/89745
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