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Journal article

True optical resolution beyond the Rayleigh limit achieved by standing wave illumination.

  • Frohn JT Nanotechnology Group, Institute of Robotics, Swiss Federal Institute of Technology Zurich, Tannenstrasse 3, CH-8092 Zurich, Switzerland.
  • Knapp HF
  • Stemmer A
  • 2000-06-07
Published in:
  • Proceedings of the National Academy of Sciences of the United States of America. - 2000
Animals
Humans
Microscopy, Fluorescence
Sensitivity and Specificity
English During the last decade, various efforts have been undertaken to enhance the resolution of optical microscopes, mostly because of their importance in biological sciences. Herein, we describe a method to increase the resolution of fluorescence microscopy by illuminating the specimen with a mesh-like interference pattern of a laser source and electronic postprocessing of the images. We achieve 100-nm optical resolution, an improvement by a factor of more than 2 compared with standard fluorescence microscopy and of 1.5 compared with confocal scanning.
Language
  • English
Open access status
bronze
Identifiers
Persistent URL
https://sonar.rero.ch/global/documents/66918
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