Meropenem/colistin synergy testing for multidrug-resistant Acinetobacter baumannii strains by a two-dimensional gradient technique applicable in routine microbiology.
- van Belkum A Microbiology R&D, Microbiology Unit, bioMérieux SA, La Balme Les Grottes, France alex.vanbelkum@biomerieux.com.
- Halimi D Microbiology R&D, Microbiology Unit, bioMérieux SA, La Balme Les Grottes, France.
- Bonetti EJ Genomic Research Laboratory, Service of Infectious Diseases, Geneva University Hospital (HUG), Geneva, Switzerland.
- Renzi G Bacteriology Laboratory, Service of Infectious Diseases, Geneva University Hospital (HUG), Geneva, Switzerland.
- Cherkaoui A Bacteriology Laboratory, Service of Infectious Diseases, Geneva University Hospital (HUG), Geneva, Switzerland.
- Sauvonnet V Microbiology R&D, Microbiology Unit, bioMérieux SA, La Balme Les Grottes, France.
- Martelin R Microbiology R&D, Microbiology Unit, bioMérieux SA, La Balme Les Grottes, France.
- Durand G Microbiology R&D, Microbiology Unit, bioMérieux SA, La Balme Les Grottes, France.
- Chatellier S Microbiology R&D, Microbiology Unit, bioMérieux SA, La Balme Les Grottes, France.
- Zambardi G Microbiology R&D, Microbiology Unit, bioMérieux SA, La Balme Les Grottes, France.
- Engelhardt A AB BIODISK Research Laboratories, Solna, Sweden Franchise Identification/Antimicrobial Susceptibility Testing, Microbiology Unit, bioMérieux Inc., St Louis, MO, USA.
- Karlsson Å Microbiology R&D, Microbiology Unit, bioMérieux SA, La Balme Les Grottes, France AB BIODISK Research Laboratories, Solna, Sweden.
- Schrenzel J Genomic Research Laboratory, Service of Infectious Diseases, Geneva University Hospital (HUG), Geneva, Switzerland Bacteriology Laboratory, Service of Infectious Diseases, Geneva University Hospital (HUG), Geneva, Switzerland.
- 2014-09-21
Published in:
- The Journal of antimicrobial chemotherapy. - 2015
antibiotic resistance
susceptibility testing
two-dimensional Etest
Acinetobacter baumannii
Anti-Bacterial Agents
Colistin
Drug Resistance, Multiple, Bacterial
Drug Synergism
Meropenem
Microbial Sensitivity Tests
Thienamycins
English
OBJECTIVES
Precise assessment of potential therapeutic synergy, antagonism or indifference between antimicrobial agents currently depends on time-consuming and hard-to-standardize in vitro chequerboard titration methods. We here present a method based on a novel two-dimensional antibiotic gradient technique named Xact™.
METHODS
We used a test comprising a combination of perpendicular gradients of meropenem and colistin in a single quadrant. We compared test outcomes with those obtained with classical chequerboard microbroth dilution testing in a study involving 27 unique strains of multidrug-resistant Acinetobacter baumannii from diverse origins.
RESULTS
We were able to demonstrate 92% concordance between the new technology and classical chequerboard titration using the A. baumannii collection. Two strains could not be analysed by Xact™ due to their out-of-range MIC of meropenem (>128 mg/L).
CONCLUSIONS
The new test was shown to be diagnostically useful, easy to implement and less labour intensive than the classical method.
Precise assessment of potential therapeutic synergy, antagonism or indifference between antimicrobial agents currently depends on time-consuming and hard-to-standardize in vitro chequerboard titration methods. We here present a method based on a novel two-dimensional antibiotic gradient technique named Xact™.
METHODS
We used a test comprising a combination of perpendicular gradients of meropenem and colistin in a single quadrant. We compared test outcomes with those obtained with classical chequerboard microbroth dilution testing in a study involving 27 unique strains of multidrug-resistant Acinetobacter baumannii from diverse origins.
RESULTS
We were able to demonstrate 92% concordance between the new technology and classical chequerboard titration using the A. baumannii collection. Two strains could not be analysed by Xact™ due to their out-of-range MIC of meropenem (>128 mg/L).
CONCLUSIONS
The new test was shown to be diagnostically useful, easy to implement and less labour intensive than the classical method.
- Language
-
- English
- Open access status
- bronze
- Identifiers
-
- DOI 10.1093/jac/dku342
- PMID 25239465
- Persistent URL
- https://sonar.rero.ch/global/documents/50971
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