Journal article
Hepatocyte nuclear factor 1 negatively regulates amylin gene expression.
- 2003-10-03
Published in:
- Biochemical and biophysical research communications. - 2003
Amyloid
Animals
Binding Sites
COS Cells
Cell Line, Tumor
DNA-Binding Proteins
Gene Expression Regulation
Hepatocyte Nuclear Factor 1
Hepatocyte Nuclear Factor 1-alpha
Hepatocyte Nuclear Factor 1-beta
Islet Amyloid Polypeptide
Nuclear Proteins
Pancreas
Promoter Regions, Genetic
Rats
Transcription Factors
English
Maturity-onset diabetes of the young (MODY) is a monogenic subtype of Type 2 diabetes, defined as having an early age of onset, with a dominant inheritance pattern. Hepatocyte nuclear factor 1 (HNF1), which is encoded by the MODY3 gene, has been shown to bind the insulin promoter. Since the promoters of three pancreas-specific genes involved in glucose homeostasis-insulin, glucokinase, and amylin bind similar transcription factors, we were interested in whether HNF1 could also regulate amylin expression. In the present study, we used the electrophoretic mobility shift assay, to demonstrate that the HNF1 transcription factor can specifically bind to the amylin promoter. Moreover, co-transfection of an HNF1 expression vector with an amylin-CAT reporter plasmid decreased the activity of the amylin promoter by 85%. These data support the hypothesis that the amylin gene is regulated by HNF1 in a negative manner and may explain partially how HNF1 mutations result in diabetes.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1016/j.bbrc.2003.09.046
- PMID 14521933
- Persistent URL
- https://sonar.rero.ch/global/documents/4674
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