Back
Fulltext
Journal article

Nucleotide excision repair efficiency in quiescent human fibroblasts is modulated by circadian clock

csal

  • Bee, Leonardo Department of Biology, University of Padova, Padova 35131, Italy
  • Marini, Selena Department of Biology, University of Padova, Padova 35131, Italy
  • Pontarin, Giovanna Department of Biology, University of Padova, Padova 35131, Italy
  • Ferraro, Paola Department of Biology, University of Padova, Padova 35131, Italy
  • Costa, Rodolfo Department of Biology, University of Padova, Padova 35131, Italy
  • Albrecht, Urs Department of Biology, Unit for Biochemistry, University of Fribourg, Fribourg CH-1700, Switzerland
  • Celotti, Lucia Department of Biology, University of Padova, Padova 35131, Italy
Show more…
    2015
Published in:
  • Nucleic Acids Research. - Oxford University Press. - 2015, vol. 43, no. 4, p. 2126-2137
English The efficiency of Nucleotide Excision Repair (NER)process is crucial for maintaining genomic integrity because in many organisms, including humans, it represents the only system able to repair a wide range of DNA damage. The aim of the work was to investigate whether the efficiency of the repair of photoproducts induced by UV-light is affected by the circadian phase at which irradiation occurred. NER activity has been analyzed in human quiescent fibroblasts (in the absence of the cell cycle effect), in which circadian rhythmicity has been synchronized with a pulse of dexamethasone. Our results demonstrate that both DNA damage induction and repair efficiency are strictly dependent on the phase of the circadian rhythm at which the cells are UV-exposed. Furthermore, the differences observed between fibroblasts irradiated at different circadian times (CTs) are abolished when the clock is obliterated. In addition, we observe that chromatin structure is regulated by circadian rhythmicity. Maximal chromatin relaxation occurred at the same CT when photoproduct formation and removal were highest. Our data suggest that the circadian clock regulates both the DNA sensitivity to UV damage and the efficiency of NER by controlling chromatin condensation mainly through histone acetylation
Collections
Language
  • English
Classification
Medicine
License
License undefined
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
Identifiers
  • RERO DOC 289872
  • SWISSBIB (NATIONALLICENCE)oxford-10.1093/nar/gkv081
  • DOI 10.1093/nar/gkv081
Persistent URL
https://sonar.rero.ch/global/documents/305402
Statistics
Document views: 14 File downloads:
  • gkv081.pdf: 0