Journal article
The Cre/Lox system to assess the development of the mouse brain.
- Kratochwil CF Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
- Rijli FM
- 2013-09-20
Published in:
- Methods in molecular biology (Clifton, N.J.). - 2014
Animal Husbandry
Animals
Base Sequence
Brain
Female
Gene Knockout Techniques
Genetic Engineering
Genotyping Techniques
Integrases
Male
Mice
Recombination, Genetic
English
Cre-mediated recombination has become a powerful tool to confine gene deletions (conditional knockouts) or overexpression of genes (conditional knockin/overexpression). By spatiotemporal restriction of genetic manipulations, major problems of classical knockouts such as embryonic lethality can be circumvented. Furthermore Cre-mediated recombination has broad applicability in the analysis of the cellular behavior of subpopulations and cell types as well as for genetic fate mapping. This chapter will give an overview about applications for the Cre/LoxP system and their execution.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1007/978-1-62703-655-9_20
- PMID 24048942
- Persistent URL
- https://sonar.rero.ch/global/documents/298995
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