Imaging cellular ultrastructures using expansion microscopy (U-ExM).
- Gambarotto D Department of Cell Biology, Sciences III, University of Geneva, Geneva, Switzerland.
- Zwettler FU Department of Biotechnology and Biophysics, Biocenter, University of Würzburg, Würzburg, Germany.
- Le Guennec M Department of Cell Biology, Sciences III, University of Geneva, Geneva, Switzerland.
- Schmidt-Cernohorska M Department of Cell Biology, Sciences III, University of Geneva, Geneva, Switzerland.
- Fortun D Signal Processing Core of Center for Biomedical Imaging (CIBM-SP), EPFL, Lausanne, Switzerland.
- Borgers S Department of Cell Biology, Sciences III, University of Geneva, Geneva, Switzerland.
- Heine J Abberior Instruments GmbH, Göttingen, Germany.
- Schloetel JG Abberior Instruments GmbH, Göttingen, Germany.
- Reuss M Abberior Instruments GmbH, Göttingen, Germany.
- Unser M Ecole Polytechnique Fédérale de Lausanne (EPFL), Biomedical Imaging Group, Lausanne, Switzerland.
- Boyden ES Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
- Sauer M Department of Biotechnology and Biophysics, Biocenter, University of Würzburg, Würzburg, Germany. m.sauer@uni-wuerzburg.de.
- Hamel V Department of Cell Biology, Sciences III, University of Geneva, Geneva, Switzerland. virginie.hamel@unige.ch.
- Guichard P Department of Cell Biology, Sciences III, University of Geneva, Geneva, Switzerland. paul.guichard@unige.ch.
- 2018-12-19
Published in:
- Nature methods. - 2019
English
Determining the structure and composition of macromolecular assemblies is a major challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion microscopy that allows the visualization of preserved ultrastructures by optical microscopy. This method allows for near-native expansion of diverse structures in vitro and in cells; when combined with super-resolution microscopy, it unveiled details of ultrastructural organization, such as centriolar chirality, that could otherwise be observed only by electron microscopy.
- Language
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- English
- Open access status
- green
- Identifiers
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- DOI 10.1038/s41592-018-0238-1
- PMID 30559430
- Persistent URL
- https://sonar.rero.ch/global/documents/232826
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