BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs.
- Simão FA Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, Switzerland.
- Waterhouse RM Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, Switzerland.
- Ioannidis P Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, Switzerland.
- Kriventseva EV Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, Switzerland.
- Zdobnov EM Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, Switzerland.
- 2015-06-11
Published in:
- Bioinformatics (Oxford, England). - 2015
Animals
Computational Biology
Gene Dosage
Genome
Genomics
Humans
Molecular Sequence Annotation
Software
English
MOTIVATION
Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50.
RESULTS
We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO.
AVAILABILITY AND IMPLEMENTATION
Software implemented in Python and datasets available for download from http://busco.ezlab.org.
CONTACT
evgeny.zdobnov@unige.ch
SUPPLEMENTARY INFORMATION
Supplementary data are available at Bioinformatics online.
Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50.
RESULTS
We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO.
AVAILABILITY AND IMPLEMENTATION
Software implemented in Python and datasets available for download from http://busco.ezlab.org.
CONTACT
evgeny.zdobnov@unige.ch
SUPPLEMENTARY INFORMATION
Supplementary data are available at Bioinformatics online.
- Language
-
- English
- Open access status
- bronze
- Identifiers
-
- DOI 10.1093/bioinformatics/btv351
- PMID 26059717
- Persistent URL
- https://sonar.rero.ch/global/documents/21505
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