Journal article
Good agreement between capillary and venous sampling for lectin pathway proteins.
- Schlapbach LJ Neonatal and Pediatric Intensive Care Unit, Department of Pediatrics, University of Berne, Berne, Switzerland. luregn.schlapbach@mater.org.au
- Woerner A
- Thiel S
- Ammann RA
- Aebi C
- Nelle M
- Jensenius JC
- 2012-07-04
Published in:
- Immunobiology. - 2013
Capillaries
Complement Fixation Tests
Complement Pathway, Mannose-Binding Lectin
Female
Humans
Infant, Newborn
Male
Specimen Handling
Veins
English
BACKGROUND
The lectin pathway of complement activation, in particular mannose-binding lectin (MBL), has been extensively investigated over recent years. So far, studies were exclusively based on venous samples. The aim of this study was to investigate whether measurements of lectin pathway proteins obtained by capillary sampling are in agreement with venous samples.
METHODS
Prospective study including 31 infants that were admitted with suspected early-onset sepsis. Lectin pathway proteins were measured in simultaneously obtained capillary and venous samples. Bland-Altman plots of logarithmized results were constructed, and the mean capillary to venous ratios (ratiocap/ven) were calculated with their 95% confidence intervals (CI).
RESULTS
The agreement between capillary and venous sampling was very high for MBL (meanratiocap/ven, 1.01; 95% CI, 0.85-1.19). Similarly, high agreement was observed for H-ficolin (meanratiocap/ven, 1.02; 95% CI, 0.72-1.44), MASP-2 (1.04; 0.59-1.84), MASP-3 (0.96; 0.71-1.28), and MAp44 (1.01; 0.82-1.25), while the agreement was moderate for M-ficolin (meanratiocap/ven, 0.78; 95% CI, 0.27-2.28).
CONCLUSIONS
The results of this study show an excellent agreement between capillary and venous samples for most lectin pathway proteins. Except for M-ficolin, small volume capillary samples can thus be used when assessing lectin pathway proteins in neonates and young children.
The lectin pathway of complement activation, in particular mannose-binding lectin (MBL), has been extensively investigated over recent years. So far, studies were exclusively based on venous samples. The aim of this study was to investigate whether measurements of lectin pathway proteins obtained by capillary sampling are in agreement with venous samples.
METHODS
Prospective study including 31 infants that were admitted with suspected early-onset sepsis. Lectin pathway proteins were measured in simultaneously obtained capillary and venous samples. Bland-Altman plots of logarithmized results were constructed, and the mean capillary to venous ratios (ratiocap/ven) were calculated with their 95% confidence intervals (CI).
RESULTS
The agreement between capillary and venous sampling was very high for MBL (meanratiocap/ven, 1.01; 95% CI, 0.85-1.19). Similarly, high agreement was observed for H-ficolin (meanratiocap/ven, 1.02; 95% CI, 0.72-1.44), MASP-2 (1.04; 0.59-1.84), MASP-3 (0.96; 0.71-1.28), and MAp44 (1.01; 0.82-1.25), while the agreement was moderate for M-ficolin (meanratiocap/ven, 0.78; 95% CI, 0.27-2.28).
CONCLUSIONS
The results of this study show an excellent agreement between capillary and venous samples for most lectin pathway proteins. Except for M-ficolin, small volume capillary samples can thus be used when assessing lectin pathway proteins in neonates and young children.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1016/j.imbio.2012.06.001
- PMID 22749981
- Persistent URL
- https://sonar.rero.ch/global/documents/192590
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