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T cell–independent development and induction of somatic hypermutation in human IgM+IgD+CD27+ B cells

  • Scheeren, Ferenc A. Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, Netherlands
  • Nagasawa, Maho Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, Netherlands
  • Weijer, Kees Netherlands Cancer Institute–Antoni van Leeuwenhoek Ziekenhuis, 1066 CX Amsterdam, Netherlands
  • Cupedo, Tom Erasmus University Medical Center, 3000 CA Rotterdam, Netherlands
  • Kirberg, Jörg University of Lausanne, Department of Biochemistry, 1066 Epalinges, Switzerland
  • Legrand, Nicolas Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, Netherlands
  • Spits, Hergen Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, Netherlands
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  • 2008-8-11
Published in:
  • Journal of Experimental Medicine. - Rockefeller University Press. - 2008, vol. 205, no. 9, p. 2033-2042
Immunology
Immunology and Allergy
English IgM+IgD+CD27+ B cells from peripheral blood have been described as circulating marginal zone B cells. It is still unknown when and where these cells develop. These IgM+IgD+CD27+ B cells exhibit somatic hypermutations (SHMs) in their B cell receptors, but the exact nature of the signals leading to induction of these SHMs remains elusive. Here, we show that IgM+IgD+CD27+ B cells carrying SHMs are observed during human fetal development. To examine the role of T cells in human IgM+IgD+CD27+ B cell development we used an in vivo model in which Rag2−/−γC−/− mice were repopulated with human hematopoietic stem cells. Using Rag2−/−γC−/− mice on a Nude background, we demonstrated that development and induction of SHMs of human IgM+IgD+CD27+ B cells can occur in a T cell–independent manner.
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  • English
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hybrid
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https://sonar.rero.ch/global/documents/1857
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