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A new approach for cryofixation by high-pressure freezing.
Journal article

A new approach for cryofixation by high-pressure freezing.

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  • 2001-09-14
Published in:
  • Journal of microscopy. - 2001
Animals
Atmospheric Pressure
Freeze Substitution
Freezing
Liver
Microscopy, Electron
Microtomy
Peripheral Nerves
Plant Leaves
Rats
Temperature
Tissue Fixation
Yeasts
English A newly designed high-pressure freezing machine for cryofixation was established and tested (Leica EMPACT), based on ideas originally proposed by Moor & Riehle in 1968. The new machine, essentially an improved version of our prototype, pressurizes the sample to 2000 bar in a small container (using methylcyclohexane as hydraulic fluid) and at the same time cools the outer surface of the container with a jet of liquid nitrogen. The advantage of this approach is that the machine uses little liquid nitrogen and can be built small and light. The machine is able to vitrify and freeze well a variety of specimens, for example, plant leaves, yeast cells, liver or nerve tissue (more samples are shown at: http://www.ana.unibe.ch/empact). Cooling efficiency is the same as in the traditional machines that use liquid nitrogen to pressurize and simultaneously cool the sample.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.rero.ch/global/documents/182604
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