No more non-model species: the promise of next generation sequencing for comparative immunology.
- Dheilly NM CNRS, UMR 5244, Ecologie et Evolution des Interactions (2EI), Perpignan F-66860, France; Université de Perpignan Via Domitia, Perpignan F-66860, France. Electronic address: ndheilly@hotmail.fr.
- Adema C Center for Evolutionary and Theoretical Immunology, Biology Department, University of New Mexico, Albuquerque, NM 87131, USA.
- Raftos DA Department of Biological Sciences, Macquarie University, North Ryde, NSW 2109, Australia.
- Gourbal B CNRS, UMR 5244, Ecologie et Evolution des Interactions (2EI), Perpignan F-66860, France; Université de Perpignan Via Domitia, Perpignan F-66860, France.
- Grunau C CNRS, UMR 5244, Ecologie et Evolution des Interactions (2EI), Perpignan F-66860, France; Université de Perpignan Via Domitia, Perpignan F-66860, France.
- Du Pasquier L University of Basel, Institute of Zoology and Evolutionary Biology, Basel, Switzerland.
- 2014-02-11
Published in:
- Developmental and comparative immunology. - 2014
Association studies
Diversification
Epigenetic
Gene expression
Immune system
Transcriptome sequencing
Allergy and Immunology
Animals
Epigenesis, Genetic
Gene Expression Profiling
Genome-Wide Association Study
High-Throughput Nucleotide Sequencing
Host-Pathogen Interactions
Humans
Models, Animal
Sequence Analysis, DNA
Transcriptome
English
Next generation sequencing (NGS) allows for the rapid, comprehensive and cost effective analysis of entire genomes and transcriptomes. NGS provides approaches for immune response gene discovery, profiling gene expression over the course of parasitosis, studying mechanisms of diversification of immune receptors and investigating the role of epigenetic mechanisms in regulating immune gene expression and/or diversification. NGS will allow meaningful comparisons to be made between organisms from different taxa in an effort to understand the selection of diverse strategies for host defence under different environmental pathogen pressures. At the same time, it will reveal the shared and unique components of the immunological toolkit and basic functional aspects that are essential for immune defence throughout the living world. In this review, we argue that NGS will revolutionize our understanding of immune responses throughout the animal kingdom because the depth of information it provides will circumvent the need to concentrate on a few "model" species.
- Language
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- English
- Open access status
- green
- Identifiers
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- DOI 10.1016/j.dci.2014.01.022
- PMID 24508980
- Persistent URL
- https://sonar.rero.ch/global/documents/17633
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