Journal article
Monosynaptic rabies virus reveals premotor network organization and synaptic specificity of cholinergic partition cells.
- Stepien AE Department of Cell Biology, University of Basel, Biozentrum, Klingelbergstrasse 70, 4056 Basel, Switzerland.
- Tripodi M
- Arber S
- 2010-11-03
Published in:
- Neuron. - 2010
Animals
Efferent Pathways
GTP-Binding Proteins
Immunohistochemistry
Interneurons
Mice
Mice, Inbred C57BL
Motor Neurons
Muscle, Skeletal
Parasympathetic Nervous System
Presynaptic Terminals
Rabies
Rabies virus
Spinal Cord
Synapses
English
Movement is the behavioral output of neuronal activity in the spinal cord. Motor neurons are grouped into motor neuron pools, the functional units innervating individual muscles. Here we establish an anatomical rabies virus-based connectivity assay in early postnatal mice. We employ it to study the connectivity scheme of premotor neurons, the neuronal cohorts monosynaptically connected to motor neurons, unveiling three aspects of organization. First, motor neuron pools are connected to segmentally widely distributed yet stereotypic interneuron populations, differing for pools innervating functionally distinct muscles. Second, depending on subpopulation identity, interneurons take on local or segmentally distributed positions. Third, cholinergic partition cells involved in the regulation of motor neuron excitability segregate into ipsilaterally and bilaterally projecting populations, the latter exhibiting preferential connections to functionally equivalent motor neuron pools bilaterally. Our study visualizes the widespread yet precise nature of the connectivity matrix for premotor interneurons and reveals exquisite synaptic specificity for bilaterally projecting cholinergic partition cells.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1016/j.neuron.2010.10.019
- PMID 21040847
- Persistent URL
- https://sonar.rero.ch/global/documents/12392
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