Journal article
Halothane metabolism: Kupffer cells carry and partially process trifluoroacetylated protein adducts.
- Christen U Dept. of Pharmacology, Biocenter of the University, Basel, Switzerland.
- Bürgin M
- Gut J
- 1991-02-28
Published in:
- Biochemical and biophysical research communications. - 1991
Animals
Biotransformation
Blotting, Western
Cell Separation
Cells, Cultured
Electrophoresis, Polyacrylamide Gel
Halothane
Kupffer Cells
Liver
Male
Molecular Weight
Protein Binding
Proteins
Rats
Rats, Inbred Strains
English
Kupffer cells, prepared 18 h after pretreatment of rats with a single dose of halothane, did carry TFA-adducts which were recognized on Western blots by a anti-TFA-antibody. Based on apparent molecular weight, the pattern of the major TFA-adducts within Kupffer cells was similar to that observed in hepatocytes. When kept in primary culture, Kupffer cells processed TFA-adducts of apparent molecular weight of 220 kD, 110 kD and 74 kD within 24 or 48 h; in contrast, other TFA-adducts were persistent for at least 48 h in Kupffer cells. The data suggest a role for Kupffer cells in processing of chemically altered proteins in the liver.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1016/s0006-291x(05)81228-8
- PMID 1998510
- Persistent URL
- https://sonar.rero.ch/global/documents/103855
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