Journal article
Phage selection of bicyclic peptides.
- Rentero Rebollo I Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.
- Heinis C
- 2013-01-15
Published in:
- Methods (San Diego, Calif.). - 2013
English
Bicyclic peptides are small, constrained peptides that can bind with high affinity and selectivity to protein targets. Their small size provides a number of advantages over larger protein-based ligands, including access to chemical synthesis, better tissue penetration, and a wider choice of application routes. Bicyclic peptide ligands can be identified using phage display technology with moderate effort and cost. Here we provide step-by-step protocols for the isolation of bicyclic peptide ligands using phage display. These protocols have been successfully used in our laboratory for the generation of high-affinity binders to a variety of protein targets. We describe library generation, affinity selection and ligand characterization, and provide troubleshooting advice concerning frequent problems.
- Language
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- English
- Open access status
- closed
- Identifiers
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- DOI 10.1016/j.ymeth.2012.12.008
- PMID 23313750
- Persistent URL
- https://sonar.rero.ch/global/documents/10134
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